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胚胎/IPS干細(xì)胞無(wú)血清培養(yǎng)基產(chǎn)品信息:
NutriStem® hESC XF, Xeno-Free medium for human ES & iPS Cell culture, with HSA
貨號(hào):05-100-1A/B，人類胚胎干細(xì)胞無(wú)血清培養(yǎng)基（含人血清白蛋白）
AF NutriStem® hESC XF, Xeno-Free medium for human ES & iPS Cell culture, without HSA
貨號(hào): 05-102-1A/B，人類胚胎干細(xì)胞無(wú)血清培養(yǎng)基（不含人血清白蛋白）
人類胚胎干細(xì)胞研究是當(dāng)前zui熱門(mén)的科研課題之一，經(jīng)由無(wú)限制的增殖及適當(dāng)?shù)恼T導(dǎo)分化后，胚胎細(xì)胞干細(xì)胞可分化成人體中各種類型的細(xì)胞，如神經(jīng)細(xì)胞、心肌細(xì)胞及軟骨細(xì)胞，這些特點(diǎn)造就胚胎干細(xì)胞未來(lái)在人類醫(yī)學(xué)健康應(yīng)用上不可估量的前景。
傳統(tǒng)干細(xì)胞培養(yǎng)過(guò)程中必需添加的異種動(dòng)物（非人類）成分，如牛血清或豬血清，使之無(wú)法*排除人畜共染病毒、朊病毒（Prion）及支原體污染細(xì)胞 的可能性，這是造成人類干細(xì)胞進(jìn)入臨床應(yīng)用的主要障礙之一。經(jīng)由使用成分明確、全合成的無(wú)血清干細(xì)胞培養(yǎng)基可有效避免人畜共染疾病的問(wèn)題，同時(shí)全合成培養(yǎng) 基無(wú)批間差異，培養(yǎng)條件容易保持*，實(shí)驗(yàn)的重復(fù)性大為提高。應(yīng)用無(wú)血清干細(xì)胞培養(yǎng)基可為干細(xì)胞在心血管疾病、神經(jīng)性退化及癌癥的臨床治療方面打開(kāi)了一扇 大門(mén)。
為了滿足科研與生物醫(yī)藥企業(yè)的需求，BI公司與以色列國(guó)家科學(xué)研究院（Technion-Israel Institute of Technology）的Dr. Itskovitz共同合作開(kāi)發(fā)了一代、擁有自主知識(shí)產(chǎn)權(quán)的人類胚胎干細(xì)胞（hESC）和誘導(dǎo)胚胎干細(xì)胞（iPSC）無(wú)血清*培養(yǎng)基—— NutriStem®。
Dr. Joseph Itskovitz己在Stem Cells, Stem Cell Dev, PNAS, Nature及Science等雜志發(fā)表近百篇文章，并有三項(xiàng)胚胎干細(xì)胞培養(yǎng)與分化誘導(dǎo)，同時(shí)也是1998年*篇分離人類胚胎干細(xì)胞論文的共同作者，現(xiàn)任職于以色列國(guó)家科學(xué)研究院Rambam Medical Center。美國(guó)國(guó)家衛(wèi)生研究院干細(xì)胞庫(kù)中批準(zhǔn)用于科研的22株人類胚胎干細(xì)胞由其團(tuán)隊(duì)提供了8株。
Embryonic stem cell lines derived from human blastocysts. J. A. Thomson, J. Itskovitz-Eldor, et. al., Science. Vol. 282, no. 5391, 1145-1147. 1998.
NutriStem® hESC XF, Xeno-Free medium for human ES & iPS Cell culture, with HSA
貨號(hào):05-100-1A/B，人類胚胎干細(xì)胞無(wú)血清培養(yǎng)基（含人血清白蛋白）
開(kāi)瓶即可使用的*培養(yǎng)基，不含異源動(dòng)物成分，添加了醫(yī)療級(jí)的人血清白蛋白（HSA：Human Serum Albumin），以適用于無(wú)滋養(yǎng)層培養(yǎng)法（Feeder-Free），如Matrigel™或conditioned medium培養(yǎng)。
AF NutriStem® hESC XF, Xeno-Free medium for human ES & iPS Cell culture, without HSA
貨號(hào): 05-102-1A/B，人類胚胎干細(xì)胞無(wú)血清培養(yǎng)基（不含人血清白蛋白）
開(kāi)瓶即可使用的*培養(yǎng)基，不含任何動(dòng)物來(lái)源成分，適用于鼠胚胎纖維原細(xì)胞（Mouse Embryonic Fibroblasts-MEF）及人包皮纖維原細(xì)胞（Human Foreskin Fibroblasts-HFF）滋養(yǎng)層培養(yǎng)法。
NutriStem®已發(fā)表參考文獻(xiàn)：
1. Human and mouse adipose-derived cells support feeder-independent induction of pluripotent stem cells. Sugii et al. PNAS. Vol. 107, No 8, 3558-3563, 2009.
2. Current technology for the derivation of pluripotent stem cell lines from human embryos. Hasegawa et al., Cell Stem Cell. Vol. 6, 521-531, 2010.
3. Progress and challenges in optimiztion of human pluripotent stem cell culture. Ge et al., Current Stem Cell research & Therapy. Vol. 5, 207-214, 2010.
4. Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA. Warren et al., Cell Stem Cell. Vol. 7, 1-13, 2010.
5. Fibrin microbeads (FMB) loaded with mesenchymal cells support their long term survival while sealed at room temperature. Gorodetsky et al. Tissue Engineering Part C: Methods. Online Ahead of Editing, March 2011.
6. Site-specific gene correction of a point mutation in human iPS cells derived from an adult patient with sickle cell disease. Zou, et al. Blood. August 2011.
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