咨詢：  （重慶） （貴陽）

Anti-E Cadherin antibody (ab15148)抗E鈣黏蛋白抗體產(chǎn)品信息：

• Product nameAnti-E Cadherin antibody
• Description

  Rabbit polyclonal to E Cadherin

• SpecificityWe have data to indicate that this antibody may not cross react with Chicken. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
• Tested applicationsWB, ICC/IF, ELISA, ICC, IHC-P, IHC-Fr
• Species reactivity

  Reacts with: Human, Pig

• Immunogen

  Recombinant fragment: IFFCERNPKP QVINIIDADL PPNTSPFTAE LTHGASANWT IQYNDPTQES IILKPKMALE VGDYKINLKL MDNQNKDQVT TLEVSVCDCE GAAGVCRKAQ PVEAGLQI , corresponding to amino acids 600-707 of Human E Cadherin.

• Positive control
  • Skin

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: Sodium Azide
  Constituents: 10mM PBS, BSA. pH 7.4

• Concentration 500 µl at 0.2 mg/ml

• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Cell Adhesion
  • Cadherins
  • Signal Transduction
  • Signaling Pathway
  • Calcium Signaling
  • Calcium Binding Proteins
  • Cancer
  • Invasion/microenvironment
  • ECM
  • Cell adhesion
  • Cadherins
  • Developmental Biology
  • Organogenesis
  • Excretory system development
  • Kidney development

Applications

Our Abpromise guarantee covers the use of ab15148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

• FunctionCadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
  E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.
• Tissue specificityNon-neural epithelial tissues.
• Involvement in diseaseDefects in CDH1 are the cause of hereditary diffuse gastric cancer (HDGC) [MIM:137215]. An autosomal dominant cancer predisposition syndrome with increased susceptibility to diffuse gastric cancer. Diffuse gastric cancer is a malignant disease characterized by poorly differentiated infiltrating lesions resulting in thickening of the stomach. Malignant tumors start in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. Note=Heterozygous germline mutations CDH1 are responsible for familial cases of diffuse gastric cancer. Somatic mutations in the has also been found in patients with sporadic diffuse gastric cancer and lobular breast cancer.
  Defects in CDH1 are a cause of susceptibility to endometrial cancer (ENDMC) [MIM:608089].
  Defects in CDH1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
• Sequence similaritiesContains 5 cadherin domains.
• Post-translational
  modificationsDuring apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions.
• Cellular localizationCell junction. Cell membrane. Endosome. Golgi apparatus > trans-Golgi network. Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane.

• Database links

  • Entrez Gene: 999 Human
  • Entrez Gene: 100048953 Pig
  • Omim: 192090 Human
  • SwissProt: P12830 Human
  • Unigene: 461086 Human

• Alternative names

  • Arc 1 antibody
  • CADH1_HUMAN antibody
  • Cadherin 1 antibody

Anti-E Cadherin antibody images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - E Cadherin antibody (ab15148)

  Immunohistochemical staining of formalin fixed paraffin embedded human skin using ab15148.

• 

  Immunocytochemistry/ Immunofluorescence - E Cadherin antibody (ab15148)This image is courtesy of an Abreview submitted by Dr Mauricio Pinto

  ab15148 staining human MCF10A cells by ICC/IF.  Cells were fixed with paraformaldehyde and blocked using 10% serum for 30 minutes at 25 °C.  The primary antibody was diluted 1/25 in TBST and incubated for 1 hour at 25 °C.  An Alexa Fluor® 555 goat anti-rabbit was used as the secondary antibody.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - E Cadherin antibody (ab15148)This image is a courtesy of Anonymous Abreview

  ab15148 staining E Cadherin in human stomach tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before heat mediated antigen retrieval in Citrate pH 6.0 and then blocking with 5% serum for 1 hour at 23°C was performed. The primary antibody was used diluted 1/50 and incubated with sample for 1 hour at 23°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary antibody.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - E Cadherin antibody (ab15148)This image is courtesy of an anonymous Abreview

  ab15148 staining E Cadherin in Pig Cervix uteri tissue sections by IHC-P (Formaldehyde-fixed, Paraffin-embedded sections). Tissue samples were fixed with formaldehyde and blocked with 10% goat serum for 1 hour at 37°C; antigen retrieval was by heat mediation in 10mM citrate at pH 6 for 2 minutes. The sample was incubated with primary antibody (1/50) at 4°C for 12 hours. An HRP-conjugated goat polyclonal to rabbit IgG (undiluted) was used as secondary antibody.
• 

  Immunocytochemistry/ Immunofluorescence - E Cadherin antibody (ab15148)This image is courtesy of an anonymous Abreview

  ab15148 staining E cadherin in Human AGS Gastric carcinoma cultured cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilzed with 0.025% Trton X-100 in TBS and blocked with 5% serum for 1 hour at 23°C. Samples were incubated with primary antibody (1/50 in blocking buffer) for 1 hour at 23°C. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.
• 

  Immunohistochemistry (Frozen sections) - E Cadherin antibody (ab15148)This image is courtesy of an anonymous Abreview.

  ab15148 staining E Cadherin in Human AGS Gastric Carcinoma tissue sections by Immunohistochemistry (Frozen sections). The sections were acetone fixed and blocked in 5% serum for 1 hour at 23°C. The primary antibody was diluted 1/50 in blocking buffer and incubated with the sample for 1 hours at 23°C.  An HRP-conjugated Goat polyclonal to Rabbit IgG, diluted 1/200, was used as the secondary.

• 
• Western blot - E Cadherin antibody (ab15148)

  All lanes : Anti-E Cadherin antibody (ab15148) at 1/500 dilution (for 16 hours at 4°C)
  Lane 1 : Human OE33 cell - whole cell lysate
  Lane 2 : Human OE33 cell - whole cell lysate
  Lysates/proteins at 20 µg per lane.
  Secondary
  Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/1000 dilution
  developed using the ECL technique
  Performed under reducing conditions.
  Observed band size : 120 kDa

• 公司介紹：

      Abcam位于英國的劍橋科學(xué)園，成立于1998年，專門生產(chǎn)和分銷研究型抗體。主要創(chuàng)立人Jonathan Milner和實驗室同事構(gòu)思成立一間了解科研人員需要的網(wǎng)上抗體公司，理念是Abcam銷售質(zhì)量高的抗體，并提供zui全面、誠實、時新的說明書，快速的送貨服務(wù)，及高效率的顧客和服務(wù)。我們的在線目錄(szbblys.com) 已有差不多100,000種抗體和試劑，并不斷添加，供應(yīng)予百多個國家。Abcam于2005年11月在倫敦證券交易所上市，在美國馬薩諸塞州、日本東京及香港均設(shè)有分公司。

      Abcam 一直致力加強(qiáng)產(chǎn)品線，為使研究員更容易找到蛋白質(zhì)研究試劑產(chǎn)品，在2011并購了美國的MitoSciences公司，加強(qiáng)了免疫分析方面的產(chǎn)品供應(yīng)；同年也并購了英國的Ascent Scientific 公司，開展了生化試劑的供應(yīng)。在2012年，Abcam并購了美國的Epitomics 公司，成為一家有的RabMAbs® 供應(yīng)商。

      Abcam 的 目標(biāo)是給世界上的抗體建立zui大的在線目錄，為各地科學(xué)家提供*產(chǎn)品，成為各國科學(xué)界的重要伙伴。我們?yōu)樗挟a(chǎn)品提供來使客戶獲得預(yù)期的結(jié) 果。為提供高質(zhì)量的抗體來指向盡可能多的靶蛋白，我們盡所能在盡可能多的應(yīng)用和物種中檢測每種抗體。我們相信誠信才是上策，在我們的上會發(fā)布有關(guān)我們 每一種產(chǎn)品的盡可能多的信息。

  更多產(chǎn)品：

  Abcamab1132Mouse monoclonal [1718] to Thyroid Hormone Receptor100ul3996Thyroid Hormone Receptor抗體一抗

  Abcamab11320Rabbit polyclonal to gamma Tubulin - Centrosome Marker100ul3996gamma Tubulin抗體一抗

  Abcamab11321Rabbit polyclonal to gamma Tubulin - Centrosome Marker100ul3996gamma Tubulin抗體一抗

  Abcamab11322Mouse monoclonal [TUB-11] to epsilon Tubulin100ul3996epsilon Tubulin抗體一抗

  Abcamab11323Mouse monoclonal [6-11B-1] to Tubulin （acetyl K40）100ul3996Tubulin抗體一抗

  Abcamab11324Mouse monoclonal [B3] to Tubulin - Polyglutamylated100ul4274Tubulin抗體一抗

  Abcamab11325Mouse monoclonal [TUB-1A2] to Tubulin100ul3996Tubulin抗體一抗

  Abcamab11326Mouse monoclonal [100/2] to alpha Adaptin100ul3996alpha Adaptin抗體一抗

  Abcamab11327Mouse monoclonal [100/1] to AP1+2 beta1 00ul3996beta 1/2 Adaptin抗體一抗

  Abcamab11328Mouse monoclonal [100/3] to gamma Adaptin100ul3996gamma Adaptin抗體一抗

• Abcamab12793RNA polymerase II CTD repeat YSPTSPS peptide - phospho S2100ug3286RNA polymerase II CTD repeat YSPTSPS蛋白/多肽多肽

  Abcamab12795RNA polymerase II CTD repeat YSPTSPS peptide100ug2010RNA polymerase II CTD repeat YSPTSPS蛋白/多肽多肽

  Abcamab12797Rabbit polyclonal to GPCR ORF450ug3996GPCR ORF4hystemcell一抗

  Abcamab12798Rabbit polyclonal to 5-oxo-ETE GPCR50ug4940GPCR TG1019hystemcell一抗

  Abcamab1280Mouse IgG1 （HybIgG1） （Phycoerythrin） - Isotype control1ml3996Mouse IgG1hystemcell同型對照

  Abcamab12800Rabbit polyclonal to GPCR TM7SF150ug3996GPCR TM7SF1hystemcell一抗

  Abcamab12801Rabbit polyclonal to GPR5650ug3996GPR56hystemcell一抗

  Abcamab12807Rabbit polyclonal to GPCR GPR10350ug4940GPCR GPR103hystemcell一抗

  Abcamab1281Mouse IgG2a （HybIgG2a） （FITC） （HybIgG2a） - isotype control1ml3996Mouse IgG2ahystemcell同型對照

  Abcamab12815Rabbit polyclonal to GPCR GPR12850ug4940GPCR GPR128hystemcell一抗

  Abcamab12818Rabbit polyclonal to GPCR GPR2250ug4940GPCR GPR22hystemcell一抗

  Abcamab1282Mouse IgG2a monoclonal [HybIgG2a] （Phycoerythrin） - isotype control1ml3996Mouse IgG2ahystemcell同型對照

  Abcamab12820Rabbit polyclonal to GPCR GPR2750ug3996GPCR GPR27hystemcell一抗

  Abcamab12827Rabbit polyclonal to GPCR GPR4950ug5029GPCR GPR49hystemcell一抗

  Abcamab1283Mouse IgG2b （HybIgG2b） （FITC） - Isotype control100tests3996Mouse IgG2bhystemcell同型對照

  Abcamab12834Rabbit polyclonal to GPCR GPR7850ug4940GPCR GPR78hystemcell一抗

  Abcamab1284Mouse IgG2b monoclonal [HybIgG2b] （Phycoerythrin） - isotype control1ml3996Mouse IgG2bhystemcell同型對照

  Abcamab12842Rabbit polyclonal to GPCR GPRC5B50ug3996GPCR GPRC5Bhystemcell一抗

  Abcamab12843Rabbit polyclonal to GPCR GPRC5D50ug4940GPCR GPRC5Dhystemcell一抗

  Abcamab12847Rabbit polyclonal to GPR 13950ug3996GPCR LS190419hystemcell一抗

  Abcamab1285Mouse IgG1 monoclonal [HybIgG1] （FITC+Phycoerythrin） - isotype control50tests3996Mouse IgG1hystemcell同型對照

  Abcamab12855Rabbit polyclonal to GPCR MRGE50ug3996GPCR MRGEhystemcell一抗

  Abcamab12857Rabbit polyclonal to GPCR MRGX250ug3996GPCR MRGX2hystemcell一抗

  Abcamab12861Rabbit polyclonal to Casein Kinase 2 beta （phospho S209）50ul3996Casein Kinase 2 betahystemcell一抗

  Abcamab12862Rabbit polyclonal to Syk （phospho Y323）50ul3996Sykhystemcell一抗

  Abcamab12863Rabbit polyclonal to GAB1 （phospho Y627）50ul3996GAB1hystemcell一抗

  Abcamab12864Rabbit polyclonal to RPS6 （phospho S235+S236）50ul3996RPS6hystemcell一抗

  Abcamab12865Rabbit polyclonal to RPS6 （phospho S244+S247）50ul3996RPS6hystemcell一抗

  Abcamab12866Rabbit polyclonal to Cofilin （phospho S3）50ul4274Cofilinhystemcell一抗

  Abcamab12868Rabbit polyclonal to ZAP70 （phospho Y292）50ul3996ZAP70hystemcell一抗

  Abcamab12869Rabbit polyclonal to ZAP70 （phospho Y315+Y319）50ul3996ZAP70hystemcell一抗

  Abcamab1287Rabbit polyclonal to Aurora A50ug3996Aurora Ahystemcell一抗

  Abcamab12873Rabbit polyclonal to MCHR50ug4940GPCR SLC/MCH1hystemcell一抗

  Abcamab12874Rabbit polyclonal to GPR5650ug3996GPR56hystemcell一抗

  Abcamab12875Rabbit polyclonal to Aurora A100ug4418Aurora Ahystemcell一抗

  Abcamab12894Rabbit polyclonal to Mannose 6 Phosphate Receptor （Cation independent） - Late Endosome Marker100ug3996Mannose 6 Phosphate Receptor （Cation independent）hystemcell一抗

  Abcamab1291Rabbit polyclonal to BRCA1 （Agarose）100ug5029BRCA1hystemcell一抗

  Abcamab12916Rabbit polyclonal to NR1D150ug4607NR1D1hystemcell一抗

  Abcamab1292Rabbit polyclonal to BRCA2 （Agarose）50ug3996BRCA2hystemcell一抗

  Abcamab12923Rabbit polyclonal to alpha 1d Adrenergic Receptor50ug4607alpha 1d Adrenergic Receptorhystemcell一抗

  Abcamab12925Mouse monoclonal [GP-3] to RASA3100ul3996RASA3hystemcell一抗

  Abcamab12926Rabbit polyclonal to 5HT2B Receptor50ug39965HT2B Receptorhystemcell一抗

  Abcamab12929Rabbit polyclonal to Histone H2B （mono methyl K5） -ChIP Grade50ug3996Histone H2Bhystemcell一抗

  Abcamab1293Rabbit polyclonal to MKLP1 （Agarose）100ug5029MKLP1hystemcell一抗

  Abcamab12935Rabbit polyclonal to alpha 1c Adrenergic Receptor50ug4940alpha 1c Adrenergic Receptorhystemcell一抗

  Abcamab1294Rabbit polyclonal to MSH2 （Agarose）100ug3996MSH2hystemcell一抗

  Abcamab12945Rabbit polyclonal to BDKRB150ug3996BDKRB1hystemcell一抗

  Abcamab12947Rabbit polyclonal to BDKRB225ug3996BDKRB2hystemcell一抗

  Abcamab1295Rabbit polyclonal to MLH1 （Agarose）100ug5029MLH1hystemcell一抗

  Abcamab12950Rabbit polyclonal to Histone H2A （mono methyl K127）50ug2664Histone H2Ahystemcell一抗

  Abcamab12952Histone H2A peptide - mono methyl K127100ug2010Histone H2A蛋白/多肽多肽

  Abcamab12958Rabbit polyclonal to Flamingo homolog 150ug4940Flamingo homolog 1hystemcell一抗

  Abcamab1296Rabbit polyclonal to SMC1 （Agarose）100ug3885SMC1hystemcell一抗

  Abcamab12962Rabbit polyclonal to C5R150ug3996C5R1hystemcell一抗

  Abcamab12964Rabbit polyclonal to Corticotropin Releasing Factor Receptor 250ug5029Corticotropin Releasing Factor Receptor 2hystemcell一抗

  Abcamab1297Rabbit polyclonal to SMC3 （Agarose）50ug3996SMC3hystemcell一抗

  Abcamab12974Rabbit polyclonal to EMR350ug3996EMR3hystemcell一抗

  Abcamab12977Rabbit polyclonal to Endothelin A Receptor50ug4773Endothelin A Receptorhystemcell一抗

  Abcamab1298Mouse monoclonal [mAbcam 1298] to Histone H3 （di methyl K9）100ug3996Histone H3hystemcell一抗

  Abcamab12986Rabbit polyclonal to Estrogen Related Receptor beta50ug3996Estrogen Related Receptor betahystemcell一抗

  Abcamab12989Rabbit polyclonal to ETL50ug3996ETLhystemcell一抗

  Abcamab1299Mouse monoclonal [NN 2C10/1] to GM130 - Golgi Marker100ug3996GM130hystemcell一抗

  Abcamab12990Rabbit polyclonal to FPR150ug4940FPR1hystemcell一抗

  Abcamab12994Rabbit polyclonal to Frizzled 650ug4940Frizzled 6hystemcell一抗

  Abcamab13001Rabbit polyclonal to GALR150ug3996GALR1hystemcell一抗

  Abcamab13003Rabbit polyclonal to GALR350ug4940GALR3hystemcell一抗

  Abcamab13004Rabbit polyclonal to Gastric Inhibitory Polypeptide Receptor50ug4607Gastric Inhibitory Polypeptide Receptorhystemcell一抗

  Abcamab13008Aurora A peptide100ug2010Aurora A蛋白/多肽多肽

  Abcamab1301Goat polyclonal to LASP1100ug3996LASP1hystemcell一抗

  Abcamab13011Rabbit polyclonal to GnRHR50ug4940GnRHRhystemcell一抗

  Abcamab13015Rabbit polyclonal to HRH325ug3996HRH3hystemcell一抗

  Abcamab13016Rabbit polyclonal to HUNK50ug3996HUNKhystemcell一抗

  Abcamab1302Goat polyclonal to CrkL100ug3996CrkLhystemcell一抗

  Abcamab13022Rabbit polyclonal to LPHN350ug4940LPHN3hystemcell一抗

  Abcamab13025Rabbit polyclonal to GPR10850ug4940GPR108hystemcell一抗

  Abcamab13026Rabbit polyclonal to EDG250ug3996EDG2hystemcell一抗

  Abcamab13027Rabbit polyclonal to EDG225ug3996EDG2hystemcell一抗

  Abcamab13028Rabbit polyclonal to EDG450ug3996EDG4hystemcell一抗

  Abcamab13033Rabbit polyclonal to MC1 Receptor50ug4940MC1 Receptorhystemcell一抗

  Abcamab13034Rabbit polyclonal to MC4 Receptor50ug3996MC4 Receptorhystemcell一抗

  Abcamab13037Rabbit polyclonal to Metabotropic Glutamate Receptor 150ug3996Metabotropic Glutamate Receptor 1hystemcell一抗

  Abcamab1304Mouse monoclonal [10] to Vascular Endothelium100ug3996Vascular Endotheliumhystemcell一抗

  Abcamab13040Rabbit polyclonal to Metabotropic Glutamate Receptor 250ug3996Metabotropic Glutamate Receptor 2hystemcell一抗

  Abcamab13041Rabbit polyclonal to Metabotropic Glutamate Receptor 350ug3996Metabotropic Glutamate Receptor 3hystemcell一抗

  Abcamab13042Rabbit polyclonal to Metabotropic Glutamate Receptor 750ug3996Metabotropic Glutamate Receptor 7hystemcell一抗

  Abcamab11329Mouse monoclonal [AP180-I] to AP180100ul3996AP18抗體一抗

  Abcamab1133Rabbit polyclonal to GABARAP100ul3996GABARAP抗體一抗

  更多詳情，請：     

  重慶市華雅干細(xì)胞技術(shù)有限公司