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Anti-ECSIT antibody （ab21288）抗ECSIT抗體產(chǎn)品信息：
- Product nameAnti-ECSIT antibody
- Description
Rabbit polyclonal to ECSIT
- Tested applicationsICC/IF, IP, IHC-P, WB
- Species reactivity
Reacts with: Mouse, Human
- Immunogen
Synthetic peptide corresponding to 14 amino acids near the C-terminus of ECSIT (Human).
- Positive control
- human heart lysate
Properties
- FormLiquid
- Storage instructionsStore at +4°C.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: PBS Concentration 100 µg at 1 mg/ml
- Purification notesIon exchange chromatography.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- NFkB Pathway
- Immunology
- Innate Immunity
- TLR Signaling
Applications
Our Abpromise guarantee covers the use of ab21288 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | ICC/IF: Use at an assay dependent dilution. PubMed: 21163940 |
| IP | IP: Use at an assay dependent dilution. PubMed: 21163940 |
| IHC-P | IHC-P: Use a concentration of 2 µg/ml. |
| WB | WB: Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximay 42 kDa (predicted molecular weight: 49 kDa). |
Target
- FunctionAdapter protein of the Toll-like and IL-1 receptor signaling pathway that is involved in the activation of NF-kappa-B via MAP3K1. Promotes proteolytic activation of MAP3K1. Involved in the BMP signaling pathway. Required for normal embryonic development (By similarity). Required for efficient assembly of mitochondrial NADH:ubiquinone oxidoreductase.
- Sequence similaritiesBelongs to the ECSIT family.
- Cellular localizationCytoplasm. Nucleus. Mitochondrion.
Database links
- Entrez Gene: 51295 Human
- Entrez Gene: 26940 Mouse
- Omim: 608388 Human
- SwissProt: Q9BQ95 Human
- SwissProt: Q9QZH6 Mouse
- Unigene: 515146 Human
- Unigene: 425568 Mouse
Alternative names
- Ecsit antibody
- ECSIT homolog antibody
- ECSIT protein antibody
Anti-ECSIT antibody images
Western blot - ECSIT antibody (ab21288)
Lane 1 : Anti-ECSIT antibody (ab21288) at 0.5 µg/ml
Lane 2 : Anti-ECSIT antibody (ab21288) at 1 µg/ml
Lane 3 : Anti-ECSIT antibody (ab21288) at 2 µg/ml
Lane 1 : human heart lysate
Lane 2 : human heart lysate
Lane 3 : human heart lysate
Predicted band size : 49 kDa
Observed band size : 42 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ECSIT antibody (ab21288)
ab21288 at 2µg/ml staining ECSIT in mouse heart by IHC
公司介紹：
Abcam位于英國的劍橋科學(xué)園，成立于1998年，專門生產(chǎn)和分銷研究型抗體。主要創(chuàng)立人Jonathan Milner和實驗室同事構(gòu)思成立一間了解科研人員需要的網(wǎng)上抗體公司，理念是Abcam銷售質(zhì)量高的抗體，并提供zui全面、誠實、時新的說明書，快速的送貨服務(wù)，及高效率的顧客和服務(wù)。我們的在線目錄(szbblys.com) 已有差不多100,000種抗體和試劑，并不斷添加，供應(yīng)予百多個國家。Abcam于2005年11月在倫敦證券交易所上市，在美國馬薩諸塞州、日本東京及香港均設(shè)有分公司。
Abcam 一直致力加強產(chǎn)品線，為使研究員更容易找到蛋白質(zhì)研究試劑產(chǎn)品，在2011并購了美國的MitoSciences公司，加強了免疫分析方面的產(chǎn)品供應(yīng)；同年也并購了英國的Ascent Scientific 公司，開展了生化試劑的供應(yīng)。在2012年，Abcam并購了美國的Epitomics 公司，成為一家有的RabMAbs® 供應(yīng)商。
Abcam 的 目標(biāo)是給世界上的抗體建立zui大的在線目錄，為各地科學(xué)家提供*產(chǎn)品，成為各國科學(xué)界的重要伙伴。我們?yōu)樗挟a(chǎn)品提供來使客戶獲得預(yù)期的結(jié) 果。為提供高質(zhì)量的抗體來指向盡可能多的靶蛋白，我們盡所能在盡可能多的應(yīng)用和物種中檢測每種抗體。我們相信誠信才是上策，在我們的上會發(fā)布有關(guān)我們 每一種產(chǎn)品的盡可能多的信息。
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