目錄編號：05111-60

描述

單克隆抗體的特異性反應(yīng)的HIT3a的CD3 / T淋巴細(xì)胞抗原受體復(fù)合物的ε鏈。CD3復(fù)合物的TCR復(fù)雜的部分，由所有成熟T淋巴細(xì)胞和胸腺細(xì)胞系的表達(dá)。CD3增強(qiáng)的信號轉(zhuǎn)導(dǎo)的抗原識別。

與TCR復(fù)合物抗體HIT3a交叉鏈接，啟動細(xì)胞的活化和增殖，但它不能用于細(xì)胞CD3染色，由于它能夠染色只有表面CD3復(fù)合物。
Catalogue Number : 05111-60

Description

The Hit3a monoclonal antibody specifically reacts with the ε chain of the CD3/T lymphocyte antigen receptor complex. The CD3 complex is part of the TCR complex, expressed by all mature T lymphocytes and by the thymocyte lineage. CD3 enhances the antigen recognition by signal transduction.

The HIT3a antibody cross-links with the TCR complex, initiating the cellular activation and proliferation, but it cannot be used for intracellular CD3 staining, as it is able to stain only the surface CD3 complex.

Additional Information

Clone
Hit3a
Format:
PE
Applications
FC
Reactivity
Human

Isotype:
Mouse IgG2a, kappa
Research Interest: Adaptive Immunity
Cell Type: NK and NKT Cells, T Cells, Treg cells, Th17 cells
Preperation:
The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Formulation:
Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
References:

McMichael, A. J. (1987). Leucocyte typing III. Oxford University Press, Oxford. Norton AJ, Isaacson PG (1985)

Knapp W (1989) Leucocyte typing IV: white cell differentiation antigens. Oxford University Press, 1989.

Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York

Lanier, L. L., Allison, J. P., & Phillips, J. H. (1986). Correlation of cell surface antigen expression on human thymocytes by multi-color flow cytometric analysis: implications for differentiation. The Journal of Immunology, 137(8), 2501-2507.